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Home > Product > ELISA Kit > Human Protein Diaphanous Homolog 1, DIAPH1 ELISA Kit

Human Protein Diaphanous Homolog 1, DIAPH1 ELISA Kit

Human Protein Diaphanous Homolog 1, DIAPH1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Diaphanous Homolog 1

Code

E2654Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity). Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity). Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity). Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells (PubMed: 20937854, PubMed: 2183$498.00|$360.007, PubMed: 26912466). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed: 20937854, PubMed: 2183$498.00|$360.007). It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (PubMed: 20937854, PubMed: 2183$498.00|$360.007). In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed: 20937854, PubMed: 2183$498.00|$360.007). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed: 20937854, PubMed: 2183$498.00|$360.007). Plays a role in brain development (PubMed: 24781755). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity).

UniProt accession

MASS(Da)

141,347

GeneID

1729

Synonyms

DIAPH 1;DIAPH1;Diaphanous-related formin-1;DRF1;Protein diaphanous homolog 1

Gene names

DIAPH1

Research area

Others

Target protein

DIAPH1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Protein Diaphanous Homolog 1, DIAPH1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.269 2.19 2.149
2.111
6ng/ml 1.827 1.777 1.736
1.727
3ng/ml 1.263 1.269 1.228
1.275
1.5ng/ml 0.68 0.685 0.644
0.691
0.75ng/ml 0.351 0.361 0.32
0.371
0ng/ml 0.034 0.041 0
0.048

Precision

We measured random samples of E2654Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.9 0.12 6.1
Intra-Assay 2 18 5.6 0.41 7.4
Intra-Assay 3 18 1.3 0.05 4.1

Protocols

References

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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