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Home > Product > ELISA Kit > Human Dickkopf Related Protein 2, DKK2 ELISA Kit

Human Dickkopf Related Protein 2, DKK2 ELISA Kit

Human Dickkopf Related Protein 2, DKK2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dickkopf Related Protein 2

Code

E2655Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.07ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).

UniProt accession

MASS(Da)

28,447

GeneID

27123

Synonyms

Dickkopf-2;Dickkopf-related protein 2;DKK 2;DKK2;Dkk-2;hDkk-2

Gene names

DKK2

Research area

Stem Cells

Target protein

DKK2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2655Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.152 2.176 2.136
2.2
20ng/ml 1.754 1.63 1.591
1.507
10ng/ml 1.178 1.165 1.125
1.152
5ng/ml 0.63 0.64 0.6
0.65
2.5ng/ml 0.442 0.463 0.423
0.483
0ng/ml 0.043 0.04 0
0.036

Precision

We measured random samples of E2655Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 22.8 1.43 6.3
Intra-Assay 2 18 8 0.58 7.2
Intra-Assay 3 18 4.4 0.3 6.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2655Hu

    $320.00

  • 96T

    E2655Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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