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Home > Product > ELISA Kit > Bovine C-C Motif Chemokine 20, CCL20 ELISA Kit

Bovine C-C Motif Chemokine 20, CCL20 ELISA Kit

Bovine C-C Motif Chemokine 20, CCL20 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 5.09ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 5.09ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

C-C Motif Chemokine 20

Code

E2195Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

5.09ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells.

UniProt accession

MASS(Da)

10,964

GeneID

281666

Synonyms

C-C motif chemokine 20;CCL 20;CCL20;Macrophage inflammatory protein 3 alpha;MIP-3-alpha;Small-inducible cytokine A20

Gene names

CCL20

Research area

Immunology; Signal Transduction

Target protein

CCL20

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2195Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.143 2.222 2.167
2.3
600ng/L 1.576 1.605 1.55
1.634
300ng/L 1.143 1.161 1.106
1.179
150ng/L 0.697 0.7 0.645
0.703
75ng/L 0.426 0.437 0.382
0.448
0ng/L 0.063 0.055 0
0.047

Precision

We measured random samples of E2195Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 188.6 13.93 7.4
Intra-Assay 2 18 687.6 41.22 6
Intra-Assay 3 18 101.3 7.67 7.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E2195Bo

    $458.00

  • 48T

    E2195Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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