Human Golgi Phosphoprotein 3, GOLPH3 ELISA Kit

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Home > Product > ELISA Kit > Human Golgi Phosphoprotein 3, GOLPH3 ELISA Kit

Human Golgi Phosphoprotein 3, GOLPH3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.11ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Golgi Phosphoprotein 3

Code

E2724Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.11ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

UniProt accession

Q9H4A6

MASS（Da）

33,811

GeneID

64083

Synonyms

Coat protein GPP34;Golgi phosphoprotein 3;GOLPH 3;GOLPH3;MIDAS;Mitochondrial DNA absence factor

Gene names

GOLPH3

Research area

Tags & Cell Markers

Target protein

GOLPH3

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E2724Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.133	2.079	2.025
	2.024
16ng/ml	1.063	0.982	0.928
	0.901
8ng/ml	0.467	0.436	0.382
	0.405
4ng/ml	0.272	0.283	0.229
	0.294
2ng/ml	0.137	0.11	0.056
	0.084
0ng/ml	0.046	0.054	0
	0.061

Precision

We measured random samples of E2724Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	21.5	0.53	2.5
Intra-Assay	2	18	9.8	0.55	5.6
Intra-Assay	3	18	13.1	0.42	3.2

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E2724Hu

$320.00
96T

E2724Hu

$458.00

Add to Cart

Lead time: Within two weeks

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