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Home > Product > ELISA Kit > Human Klotho Beta, KLB ELISA Kit

Human Klotho Beta, KLB ELISA Kit

Human Klotho Beta, KLB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.29nmol/L

Detection range: 0.5-200nmol/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.29nmol/L

Detection range: 0.5-200nmol/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Klotho Beta

Code

E2782Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.29nmol/L

Detection range

0.5-200nmol/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity).

UniProt accession

MASS(Da)

119,808

GeneID

152831

Synonyms

BetaKlotho;Beta-klotho;BKL;KLB;Klotho beta-like protein

Gene names

KLB

Research area

Metabolism

Target protein

KLB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2782Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120nmol/L 2.06 2.089 2.021
2.117
60nmol/L 1.333 1.314 1.247
1.295
30nmol/L 0.95 0.836 0.769
0.722
15nmol/L 0.513 0.477 0.41
0.442
7.5nmol/L 0.234 0.242 0.175
0.251
0nmol/L 0.068 0.067 0
0.067

Precision

We measured random samples of E2782Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 83.2 3.48 4.2
Intra-Assay 2 18 18.4 1.09 6
Intra-Assay 3 18 51.6 2.82 5.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2782Hu

    $320.00

  • 96T

    E2782Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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