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Home > Product > ELISA Kit > Human Acyloxyacyl Hydrolase, AOAH ELISA Kit

Human Acyloxyacyl Hydrolase, AOAH ELISA Kit

Human Acyloxyacyl Hydrolase, AOAH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/L

Detection range: 0.05-20ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.025ng/L

Detection range: 0.05-20ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Acyloxyacyl Hydrolase

Code

E2817Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.025ng/L

Detection range

0.05-20ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (PubMed: 1883828, PubMed: 8089145, PubMed: 29343645). By breaking down LPS, terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses (By similarity). In peritoneal macrophages, seems to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria (By similarity).

UniProt accession

MASS(Da)

65,105

GeneID

313

Synonyms

Acyloxyacyl hydrolase;Acyloxyacyl hydrolase large subunit;Acyloxyacyl hydrolase small subunit;AOAH

Gene names

AOAH

Research area

Immunology

Target protein

AOAH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2817Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/L 2.065 2.173 2.121
2.281
6ng/L 1.908 1.904 1.851
1.901
3ng/L 1.318 1.225 1.172
1.131
1.5ng/L 0.646 0.679 0.627
0.713
0.75ng/L 0.359 0.399 0.346
0.439
0ng/L 0.043 0.053 0
0.062

Precision

We measured random samples of E2817Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.9 0.09 4.7
Intra-Assay 2 18 4 0.2 5
Intra-Assay 3 18 1.1 0.05 4.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2817Hu

    $320.00

  • 96T

    E2817Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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