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Home > Product > ELISA Kit > Human Astacin Like Metallo Endopeptidase, ASTL ELISA Kit

Human Astacin Like Metallo Endopeptidase, ASTL ELISA Kit

Human Astacin Like Metallo Endopeptidase, ASTL ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Astacin Like Metallo Endopeptidase

Code

E2833Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity).

UniProt accession

MASS(Da)

45,936

GeneID

431705

Synonyms

Astacin-like metalloendopeptidase;ASTL;Oocyte astacin;Ovastacin

Gene names

ASTL

Research area

Others

Target protein

ASTL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2833Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.273 2.218 2.174
2.163
6ng/ml 1.569 1.654 1.61
1.739
3ng/ml 1.104 1.046 1.002
0.988
1.5ng/ml 0.69 0.722 0.677
0.753
0.75ng/ml 0.402 0.401 0.356
0.4
0ng/ml 0.034 0.044 0
0.055

Precision

We measured random samples of E2833Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3 0.2 6.7
Intra-Assay 2 18 5.5 0.25 4.6
Intra-Assay 3 18 1 0.07 6.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2833Hu

    $320.00

  • 96T

    E2833Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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