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Home > Product > ELISA Kit > Human Protein Cereblon, CRBN ELISA Kit

Human Protein Cereblon, CRBN ELISA Kit

Human Protein Cereblon, CRBN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.043ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Protein Cereblon

Code

E2888Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.043ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 (Probable). Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8 (PubMed: 20223979, PubMed: 24328678, PubMed: 25043012, PubMed: 25108355). Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) Channels in excitatory neurons (PubMed: 18414909, PubMed: 29530986). Likely to function by regulating the assembly and neuronal surface expression of BK Channels via its interaction with KCNT1 (PubMed: 18414909). May also be involved in regulating anxiety-like behaviors via a BK Channel-independent mechanism (By similarity).

UniProt accession

MASS(Da)

50,546

GeneID

51185

Synonyms

CRBN;Protein cereblon

Gene names

CRBN

Research area

Neuroscience

Target protein

CRBN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2888Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.385 2.357 2.309
2.33
12ng/ml 1.784 1.859 1.811
1.934
6ng/ml 1.242 1.265 1.217
1.288
3ng/ml 0.713 0.729 0.681
0.745
1.5ng/ml 0.357 0.385 0.337
0.413
0ng/ml 0.066 0.048 0
0.031

Precision

We measured random samples of E2888Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 11.4 0.59 5.1
Intra-Assay 2 18 3.4 0.15 4.4
Intra-Assay 3 18 1.5 0.08 5.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2888Hu

    $320.00

  • 96T

    E2888Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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