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Home > Product > ELISA Kit > Human Claspin, CLSPN ELISA Kit

Human Claspin, CLSPN ELISA Kit

Human Claspin, CLSPN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Claspin

Code

E2899Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

UniProt accession

MASS(Da)

151,094

GeneID

63967

Synonyms

Claspin;CLSPN;hClaspin

Gene names

CLSPN

Research area

Epigenetics and Nuclear Signaling

Target protein

CLSPN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Claspin, CLSPN ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.225 2.196 2.166
2.167
6ng/ml 1.522 1.612 1.582
1.703
3ng/ml 1.151 1.16 1.129
1.169
1.5ng/ml 0.697 0.713 0.682
0.728
0.75ng/ml 0.481 0.477 0.447
0.474
0ng/ml 0.026 0.031 0
0.035

Precision

We measured random samples of E2899Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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