Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
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Storage
Assay time
Background
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
UniProt accession
MASS（Da）
GeneID
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Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2936Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 2.098 | 2.101 | 2.051 |
| 2.103 | |||
| 12ng/ml | 1.069 | 1.11 | 1.06 |
| 1.151 | |||
| 6ng/ml | 0.539 | 0.506 | 0.456 |
| 0.473 | |||
| 3ng/ml | 0.262 | 0.281 | 0.231 |
| 0.301 | |||
| 1.5ng/ml | 0.166 | 0.153 | 0.104 |
| 0.141 | |||
| 0ng/ml | 0.059 | 0.05 | 0 |
| 0.04 |
Precision
We measured random samples of E2936Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.2 | 0.36 | 4.4 |
| Intra-Assay | 2 | 18 | 8.3 | 0.3 | 3.6 |
| Intra-Assay | 3 | 18 | 19.5 | 0.63 | 3.2 |
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