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Home > Product > ELISA Kit > Human Cohesin Subunit Sa-1, STAG1 ELISA Kit

Human Cohesin Subunit Sa-1, STAG1 ELISA Kit

Human Cohesin Subunit Sa-1, STAG1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.99ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.99ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cohesin Subunit Sa-1

Code

E2957Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.99ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

UniProt accession

MASS(Da)

144,427

GeneID

10274

Synonyms

Cohesin subunit SA-1;SCC3 homolog 1;STAG 1;STAG1;Stromal antigen 1

Gene names

STAG1

Research area

Cell Biology

Target protein

STAG1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2957Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.172 2.228 2.158
2.283
800ng/L 1.359 1.297 1.227
1.235
400ng/L 0.78 0.746 0.676
0.712
200ng/L 0.454 0.479 0.409
0.504
100ng/L 0.305 0.313 0.243
0.322
0ng/L 0.062 0.07 0
0.078

Precision

We measured random samples of E2957Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1132.6 41.7 3.7
Intra-Assay 2 18 229.1 13.55 5.9
Intra-Assay 3 18 490.7 28.49 5.8

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2957Hu

    $320.00

  • 96T

    E2957Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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