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Home > Product > ELISA Kit > Human Spry Domain-containing Socs Box Protein 1, SPSB1 ELISA Kit

Human Spry Domain-containing Socs Box Protein 1, SPSB1 ELISA Kit

Human Spry Domain-containing Socs Box Protein 1, SPSB1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.96ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.96ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Spry Domain-Containing Socs Box Protein 1

Code

E3178Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.96ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed: 15601820, PubMed: 21199876). Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (PubMed: 21199876). Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5 (PubMed: 21199876).

UniProt accession

MASS(Da)

30,942

GeneID

80176

Synonyms

SPRY domain-containing SOCS box protein 1;SPSB 1;SPSB1;SSB-1

Gene names

SPSB1

Research area

Others

Target protein

SPSB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3178Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.25 2.179 2.148
2.109
1200ng/L 1.315 1.283 1.252
1.251
600ng/L 0.65 0.658 0.627
0.666
300ng/L 0.401 0.413 0.383
0.425
150ng/L 0.24 0.215 0.184
0.19
0ng/L 0.027 0.031 0
0.035

Precision

We measured random samples of E3178Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 758 36.85 4.9
Intra-Assay 2 18 1632.2 63.94 3.9
Intra-Assay 3 18 626.7 40.33 6.4

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3178Hu

    $320.00

  • 96T

    E3178Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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