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Home > Product > ELISA Kit > Human Polyamine-Modulated Factor 1-binding Protein 1, PMFBP1 ELISA Kit

Human Polyamine-Modulated Factor 1-binding Protein 1, PMFBP1 ELISA Kit

Human Polyamine-Modulated Factor 1-binding Protein 1, PMFBP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.58ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.58ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Polyamine-Modulated Factor 1-Binding Protein 1

Code

E3187Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.58ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for normal spermatogenesis (PubMed: 1770140, PubMed: 30032984, PubMed: 30298696). It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity (PubMed: 30032984). May also be involved in the general organization of cellular cytoskeleton (By similarity).

UniProt accession

MASS(Da)

117,494

GeneID

83449

Synonyms

PMF-1-binding protein;PMFBP 1;PMFBP1;Polyamine-modulated factor 1-binding protein 1

Gene names

PMFBP1

Research area

Epigenetics and Nuclear Signaling

Target protein

PMFBP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3187Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.073 2.103 2.054
2.133
1200ng/L 1.613 1.645 1.596
1.677
600ng/L 1.008 0.989 0.94
0.971
300ng/L 0.784 0.777 0.728
0.771
150ng/L 0.42 0.413 0.363
0.405
0ng/L 0.052 0.049 0
0.047

Precision

We measured random samples of E3187Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 177.1 11.75 6.6
Intra-Assay 2 18 573.3 43.72 7.6
Intra-Assay 3 18 813.6 41.4 5.1

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3187Hu

    $320.00

  • 96T

    E3187Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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