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Home > Product > ELISA Kit > Human Serine, Threonine-protein Kinase Nek3, NEK3 ELISA Kit

Human Serine, Threonine-protein Kinase Nek3, NEK3 ELISA Kit

Human Serine, Threonine-protein Kinase Nek3, NEK3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Serine/Threonine-Protein Kinase Nek3

Code

E3202Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.

UniProt accession

MASS(Da)

57,705

GeneID

4752

Synonyms

HSPK 36;NEK 3;NEK3;Never in mitosis A-related kinase 3;NimA-related protein kinase 3;Serine/threonine-protein kinase Nek3

Gene names

NEK3

Research area

Others

Target protein

NEK3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3202Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.565 2.525 2.483
2.486
6ng/ml 1.592 1.497 1.455
1.403
3ng/ml 0.88 0.881 0.838
0.882
1.5ng/ml 0.51 0.465 0.422
0.419
0.75ng/ml 0.23 0.226 0.183
0.222
0ng/ml 0.04 0.043 0
0.046

Precision

We measured random samples of E3202Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.7 0.09 5.4
Intra-Assay 2 18 3.9 0.22 5.8
Intra-Assay 3 18 6.4 0.21 3.3

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3202Hu

    $320.00

  • 96T

    E3202Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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