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Home > Product > ELISA Kit > Rat Mothers Against Decapentaplegic Homolog 1, SMAD1 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 1, SMAD1 ELISA Kit

Rat Mothers Against Decapentaplegic Homolog 1, SMAD1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.1ng/L

Detection range: 8.12-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 4.1ng/L

Detection range: 8.12-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Mothers against decapentaplegic homolog 1

Code

E0077Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.1ng/L

Detection range

8.12-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). Has been shown to be also activated by TGF-beta (transforming growth factor) type I receptor kinase in rat intestinal epithelial cells (IEC 4-1). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (By similarity).

UniProt accession

MASS(Da)

52,713

GeneID

25671

Synonyms

MAD homolog 1;Madh1;Mothers against decapentaplegic homolog 1;Mothers against DPP homolog 1;SMAD 1;SMAD family member 1;Smad1

Gene names

Smad1

Research area

Signal Transduction; Epigenetics and Nuclear Signaling; Cancer

Target protein

Smad1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0077Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.236 2.164 2.104
2.092
600ng/L 1.141 1.261 1.201
1.38
300ng/L 0.856 0.774 0.714
0.692
150ng/L 0.425 0.421 0.361
0.417
75ng/L 0.228 0.215 0.155
0.201
0ng/L 0.056 0.06 0
0.063

Precision

We measured random samples of E0077Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 473.55 27.46 5.8
Intra-Assay 2 18 621.38 20.43 3.29
Intra-Assay 3 18 185.5 11.93 6.43

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0077Ra

    $320.00

  • 96T

    E0077Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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