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Home > Product > ELISA Kit > Human Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Kit

Human Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Kit

Human Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.42ng/L

Detection range: 9-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.42ng/L

Detection range: 9-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Activated Leukocyte Cell Adhesion Molecule

Code

E0202Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.42ng/L

Detection range

9-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:7760007, PubMed:15496415, PubMed:15048703, PubMed:16352806, PubMed:23169771, PubMed:24945728). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity). Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

UniProt accession

MASS(Da)

65,102

GeneID

214

Synonyms

Activated leukocyte cell adhesion molecule;ALCAM;CD antigen CD166;CD166;CD166 antigen;MEMD

Gene names

ALCAM

Research area

Others

Target protein

ALCAM

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0202Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.025 2.174 2.114
2.322
600ng/L 1.548 1.522 1.462
1.495
300ng/L 0.876 0.882 0.823
0.888
150ng/L 0.552 0.488 0.428
0.423
75ng/L 0.255 0.243 0.184
0.232
0ng/L 0.07 0.059 0
0.049

Precision

We measured random samples of E0202Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 276.82 13.71 4.95
Intra-Assay 2 18 756.32 34.56 4.57
Intra-Assay 3 18 193.57 8.08 4.18

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E0202Hu

    $320.00

  • 96T

    E0202Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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