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Home > Product > ELISA Kit > Human C-type Lectin Domain Family 4 Member E, CLEC4E ELISA Kit

Human C-type Lectin Domain Family 4 Member E, CLEC4E ELISA Kit

Human C-type Lectin Domain Family 4 Member E, CLEC4E ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.33ng/L

Detection range: 5-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.33ng/L

Detection range: 5-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

C-type lectin domain family 4 member E

Code

E0229Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.33ng/L

Detection range

5-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi (PubMed:18509109, PubMed:23602766). The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions (PubMed:23602766, PubMed:24101491). Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells (By similarity). Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (By similarity). Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation (By similarity). Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue (By similarity).

UniProt accession

MASS(Da)

25,073

GeneID

26253

Synonyms

CLEC4E;CLECSF9;C-type lectin domain family 4 member E;C-type lectin superfamily member 9;Macrophage-inducible C-type lectin;MINCLE

Gene names

CLEC4E

Research area

Others

Target protein

CLEC4E

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human C-type Lectin Domain Family 4 Member E, CLEC4E ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.295 2.278 2.224
2.26
600ng/L 1.753 1.686 1.632
1.618
300ng/L 1.088 1.108 1.054
1.128
150ng/L 0.701 0.689 0.635
0.676
75ng/L 0.439 0.442 0.388
0.445
0ng/L 0.045 0.054 0
0.063

Precision

We measured random samples of E0229Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 130.78 6.09 4.65
Intra-Assay 2 18 613.62 28.78 4.69
Intra-Assay 3 18 206.33 13.33 6.46

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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