Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0277Ch are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.103 | 2.073 | 2.032 |
| 2.043 | |||
| 4ng/ml | 1.793 | 1.876 | 1.835 |
| 1.959 | |||
| 2ng/ml | 1.261 | 1.239 | 1.198 |
| 1.217 | |||
| 1ng/ml | 0.603 | 0.608 | 0.567 |
| 0.612 | |||
| 0.5ng/ml | 0.357 | 0.362 | 0.321 |
| 0.367 | |||
| 0ng/ml | 0.03 | 0.041 | 0 |
| 0.052 |
Precision
We measured random samples of E0277Ch within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.8 | 0.1 | 5.45 |
| Intra-Assay | 2 | 18 | 2.88 | 0.12 | 4.05 |
| Intra-Assay | 3 | 18 | 0.77 | 0.03 | 3.86 |
Protocols
References
Customer reviews and Q&As
