Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Bovine Legumain, LGMN ELISA Kit

Bovine Legumain, LGMN ELISA Kit

Bovine Legumain, LGMN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.63ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 48T,96T

Sensitivity: 2.63ng/L

Detection range: 5-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Legumain

Code

E0328Bo

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.63ng/L

Detection range

5-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). Required for normal lysosomal protein degradation in renal proximal tubules. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

UniProt accession

MASS(Da)

49,284

GeneID

281281

Synonyms

Asparaginyl endopeptidase;Legumain;LGMN;Protease, cysteine 1

Gene names

LGMN

Research area

Others

Target protein

LGMN

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0328Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.066 2.183 2.125
2.301
400ng/L 1.938 1.865 1.806
1.792
200ng/L 1.245 1.221 1.163
1.197
100ng/L 0.736 0.682 0.624
0.628
50ng/L 0.402 0.415 0.356
0.428
0ng/L 0.052 0.058 0
0.065

Precision

We measured random samples of E0328Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 161.63 9.36 5.79
Intra-Assay 2 18 219.99 7.5 3.41
Intra-Assay 3 18 91.02 4.99 5.49

Protocols

References

Customer reviews and Q&As

No reviews
No reviews

Associated products

  • Size

    Code

    Price

  • 48T

    E0328Bo

    $320.00

  • 96T

    E0328Bo

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up