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Home > Product > ELISA Kit > Canine Cystic Fibrosis Transmembrane Conductance Regulator, CFTR ELISA Kit

Canine Cystic Fibrosis Transmembrane Conductance Regulator, CFTR ELISA Kit

Canine Cystic Fibrosis Transmembrane Conductance Regulator, CFTR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.99ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 48T,96T

Sensitivity: 4.99ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Cystic fibrosis transmembrane conductance regulator

Code

E0340Ca

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.99ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane (By similarity). Channel activity is coupled to ATP hydrolysis. The ion channel is also permeable to HCO3-; selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Contributes to the regulation of the pH and the ion content of the epithelial fluid layer. Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7. Can inhibit the chloride channel activity of ANO1 (By similarity). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (By similarity).

UniProt accession

MASS(Da)

168,491

GeneID

492302

Synonyms

ATP-binding cassette sub-family C member 7;cAMP-dependent chloride Channel;CFTR;Channel conductance-controlling ATPase;Cystic fibrosis transmembrane conductance regulator

Gene names

CFTR

Research area

Cell Biology

Target protein

CFTR

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0340Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.237 2.227 2.186
2.217
600ng/L 1.462 1.469 1.428
1.476
300ng/L 0.606 0.661 0.62
0.716
150ng/L 0.438 0.383 0.342
0.328
75ng/L 0.17 0.186 0.145
0.202
0ng/L 0.039 0.041 0
0.043

Precision

We measured random samples of E0340Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 690.1 23.91 3.46
Intra-Assay 2 18 269.51 10.7 3.97
Intra-Assay 3 18 369.14 14.9 4.04

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0340Ca

    $350.00

  • 96T

    E0340Ca

    $475.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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