Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0387Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.356 | 2.326 | 2.256 |
| 2.295 | |||
| 1200ng/L | 1.314 | 1.26 | 1.19 |
| 1.206 | |||
| 600ng/L | 0.716 | 0.705 | 0.635 |
| 0.694 | |||
| 300ng/L | 0.541 | 0.475 | 0.405 |
| 0.409 | |||
| 150ng/L | 0.236 | 0.244 | 0.174 |
| 0.251 | |||
| 0ng/L | 0.074 | 0.07 | 0 |
| 0.065 |
Precision
We measured random samples of E0387Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 361.18 | 16.52 | 4.57 |
| Intra-Assay | 2 | 18 | 1180.96 | 31.09 | 2.63 |
| Intra-Assay | 3 | 18 | 919.17 | 53.74 | 5.85 |
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