Rabbit Podocalyxin, PCX ELISA Kit

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Blood coagulation
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Signal transduction
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Stem cells
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Home > Product > ELISA Kit > Rabbit Podocalyxin, PCX ELISA Kit

Rabbit Podocalyxin, PCX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rabbit

Product overview

Full product name

Podocalyxin

Code

E0395Rb

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.046ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rabbit

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

UniProt accession

Q28645

MASS（Da）

57,041

GeneID

100009552

Synonyms

PC;PCLP1;PCLP-1;PCX;Podocalyxin;Podocalyxin-like protein 1

Gene names

PODXL

Research area

Signal Transduction; Stem Cells; Cancer; Cardiovascular

Target protein

PCX

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0395Rb are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.059	2.124	2.081
	2.189
12ng/ml	1.401	1.277	1.234
	1.152
6ng/ml	0.658	0.718	0.676
	0.779
3ng/ml	0.425	0.413	0.37
	0.401
1.5ng/ml	0.23	0.245	0.202
	0.259
0ng/ml	0.048	0.043	0
	0.038

Precision

We measured random samples of E0395Rb within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.45	0.18	5.32
Intra-Assay	2	18	7.48	0.39	5.17
Intra-Assay	3	18	14	0.51	3.66

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E0395Rb

$320.00
96T

E0395Rb

$458.00

Add to Cart

Lead time: Within two weeks

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