Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0567Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/L | 2.164 | 1.996 | 1.955 |
| 1.829 | |||
| 240ng/L | 0.94 | 0.928 | 0.887 |
| 0.916 | |||
| 120ng/L | 0.462 | 0.484 | 0.443 |
| 0.507 | |||
| 60ng/L | 0.24 | 0.26 | 0.219 |
| 0.28 | |||
| 30ng/L | 0.14 | 0.13 | 0.089 |
| 0.121 | |||
| 0ng/L | 0.033 | 0.041 | 0 |
| 0.05 |
Precision
We measured random samples of E0567Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 370.07 | 8.92 | 2.41 |
| Intra-Assay | 2 | 18 | 296.19 | 10.62 | 3.59 |
| Intra-Assay | 3 | 18 | 126.25 | 6.56 | 5.2 |
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