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Home > Product > ELISA Kit > Rat Aquaporin 3, AQP-3 ELISA Kit

Rat Aquaporin 3, AQP-3 ELISA Kit

Rat Aquaporin 3, AQP-3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 13.92ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 13.92ng/L

Detection range: 20-7000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Aquaporin 3

Code

E0567Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

13.92ng/L

Detection range

20-7000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Aquaporin 3 is the protein product of the human AQP3 gene. It is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. Aquaporin 3 is also permeable to glycerol, ammonia, urea, and hydrogen peroxide. It is expressed in various tissues including the skin, respiratory tract, and kidneys as well as various types of cancers. In the kidney, aquaproin 3 is unresponsive to vasopressin (a.k.a. Antidiuretic Hormone), unlike Aquaporin 2. This protein is also a determinant for the GIL blood group system. Suberoylanilide hydroxamic acid (SAHA) (a HDAC inhibitor) increases expression of aquaporin-3 in normal skin cells (keratinocytes).

UniProt accession

MASS(Da)

31,384

GeneID

65133

Synonyms

31.4 kDa water Channel protein;AQP-3;Aquaglyceroporin-3;Aquaporin-3

Gene names

Aqp3

Research area

Signal Transduction

Target protein

AQP-3

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0567Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000ng/L 2.174 2.177 2.132
2.18
2000ng/L 1.905 1.853 1.808
1.801
1000ng/L 1.164 1.207 1.161
1.25
500ng/L 0.723 0.667 0.622
0.612
250ng/L 0.428 0.432 0.387
0.436
0ng/L 0.057 0.045 0
0.034

Precision

We measured random samples of E0567Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 823.02 36.1 4.39
Intra-Assay 2 18 1420.9 69.79 4.91
Intra-Assay 3 18 481.09 27.01 5.61

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0567Ra

    $320.00

  • 96T

    E0567Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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