Product name
Recombinant Human Bcl-xL protein
Code
CD02273
Biological activity
Test in Process.
Endotoxin
Less than 0.1 EU/μg of rHuBcl-xL as determined by LAL method.
Synonyms
Bcl-xL, BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S,Human
Sequence
SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEGT ESEMETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIAAWMA TYLNDHLEPW IQENGGWDTF VELYGNNAAA ESRKGQERFN R
Uniprot Accession
Molecular Weight
Approximately 23.7 kDa, a single non-glycosylated polypeptide chain containing 211 amino acids.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 5 % Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Background
Bcl-xL is a transmembrane protein located in the mitochondrial membranes of cells that are long-lived and postmitotic, such as adult brain cells. It plays a role in the signal transduction pathway of the FAS-Ligand. Bcl-xL is an anti-apoptotic protein which is a member of the Bcl-2 family which are able to form heterodimers, and this is an significant event in the regulation of apoptosis. It is involved in the survival of cancer cells. Bcl-xL is the leading monitor of apoptosis/active cell suicide. It has cell death repressor activity and therefore acts as a survival protein. The human Bcl-xL shares 97 % a.a. sequence identity with Mouse and rat Bcl-xL.
Storage
-20ºC
Research area
Cell Biology
