ABCB5 Monoclonal Antibody(11A2)

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Home > Product > Antibody > ABCB5 Monoclonal Antibody(11A2)

ABCB5 Monoclonal Antibody(11A2)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB, IHC-p, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB, IHC-p, IF, ICC

Product overview

Full product name

ATP-binding cassette sub-family B member 5

Code

BT-MCA0001

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of ABCB5

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

Uniprot accession

Human: Q2M3G0;

Molecular weight

89831

Gene ID

Human: 340273

Synonyms

ABCB5; ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5

Research area

Signal Transduction; Cancer

Target protein

ABCB5

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
WB	1:2000

Images

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1.ABCB5 Monoclonal antibody(11A2) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1.ABCB5 Monoclonal antibody(11A2)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) Hela, 2) 293T diluted at 1:2000.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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