Cyclophilin B Monoclonal Antibody(2B10)

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Home > Product > Antibody > Cyclophilin B Monoclonal Antibody(2B10)

Cyclophilin B Monoclonal Antibody(2B10)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: IF, ICC, WB, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: IF, ICC, WB, IHC-p

Product overview

Full product name

Peptidyl-prolyl cis-trans isomerase B

Code

BT-MCA0027

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Cyclophilin B

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta.

Uniprot accession

Human: P23284; Mouse: P24369; Rat: P24368;

Molecular weight

N/A

Gene ID

Human: 5479

Synonyms

PPIB; CYPB; Peptidyl-prolyl cis-trans isomerase B; PPIase B; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP

Research area

Signal transduction; Immunology

Target protein

PPIB

Recommended dilution

Application	Dilution
IF	1:50-200
IHC	1:50-300
WB	1:1000-2000

Images

Immunofluorescence analysis of Hela cell. Calmodulin Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of human-lung tissue. 1.Cyclophilin B Monoclonal antibody(2B10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1.Cyclophilin B Monoclonal antibody(2B10)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Cyclophilin B Monoclonal antibody(2B10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Cyclophilin B Monoclonal antibody(2B10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1.Cyclophilin B Monoclonal antibody(2B10) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Western blot analysis of 1) Jurkat, 2)293T, 3)Rat Liver Tissue, 4)3T3 with Cyclophilin B Mouse Monoclonal antibody diluted at 1:2.000.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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