Lamin B1 Monoclonal Antibody(7C11)

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Blood coagulation
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Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
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Home > Product > Antibody > Lamin B1 Monoclonal Antibody(7C11)

Lamin B1 Monoclonal Antibody(7C11)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ICC, IP

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IHC-p, IF, ICC, IP

Product overview

Full product name

Lamin-B1

Code

BT-MCA0057

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Recombinant Protein of Lamin-B1

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.

Uniprot accession

Human: P20700; Mouse: P14733; Rat: P70615;

Molecular weight

66408

Gene ID

Human: 4001; Mouse: 16906; Rat: 116685

Synonyms

LMNB1; LMN2; LMNB; Lamin-B1

Research area

Signal transduction; Tags & cell markers; Cancer; Cell biology

Target protein

LMNB1

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-300
IP	1:200
WB	1:2000-5000

Images

Immunofluorescence analysis of Hela cell. AMPKAlpha1/2 (phospho Thr183/172) Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Lamin B1 Monoclonal antibody(7C11) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-lung-cancer tissue. 1.Lamin B1 Monoclonal antibody(7C11)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of 1) HepG2. 2) 293T, 3) Mouse Brain Tissue, 4) Rat Brain Tissue diluted at 1:5000.

Western blot analysis of lysates from 1) HepG2. 2) 293T, 3) Mouse Brain Tissue, 4)Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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