Alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)

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Home > Product > Antibody > Alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)

Alpha-tubulin (Acetyl Lys40) Monoclonal Antibody(4A8)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Tubulin alpha-1B chain

Code

BT-MCA0084

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of α-tubulin (Acetyl Lys40)

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.,PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.,similarity:Belongs to the tubulin family.,subunit:Dimer of alpha and beta chains.,

Uniprot accession

Human: P68363; Mouse: P05213; Rat: Q6P9V9;

Molecular weight

N/A

Gene ID

Human: 7277

Synonyms

Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain

Research area

Tags & cell markers; Signal transduction

Target protein

TUBA1B

Recommended dilution

Application	Dilution
IF	1:200
IHC	1:50-100
WB	1:1000-2000

Images

Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.Alpha-tubulin (Acetyl Lys40) Monoclonal antibody(4A8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.Alpha-tubulin (Acetyl Lys40) Monoclonal antibody(4A8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1.Alpha-tubulin (Acetyl Lys40) Monoclonal antibody(4A8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-liver-cancer tissue. 1.Alpha-tubulin (Acetyl Lys40) Monoclonal antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-lung tissue. 1.Alpha-tubulin (Acetyl Lys40) Monoclonal antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-spinal-cord tissue. 1.Alpha-tubulin (Acetyl Lys40) Monoclonal antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1μM, 18 hr; +), using Acetyl- a-tubulin(Lys40) Mouse Monoclonal antibody 1:2000.

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin(Acetyl Lys40) Mouse Monoclonal antibody diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin(Acetyl Lys40) Mouse Monoclonal antibody diluted at 1:200.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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