Beta II tubulin Monoclonal Antibody(Mix)

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Home > Product > Antibody > Beta II tubulin Monoclonal Antibody(Mix)

Beta II tubulin Monoclonal Antibody(Mix)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Mouse, Rat

Applications: WB, IHC-p, IF, IP

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Mouse,Rat

Applications: WB, IHC-p, IF, IP

Product overview

Full product name

Tubulin beta-2A chain

Code

BT-MCA0087

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Beta II tubulin

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene.

Uniprot accession

Human: Q13885; Mouse: Q7TMM9; Rat: P85108;

Molecular weight

N/A

Gene ID

Human: 7280; Mouse: 22151; Rat: 498736

Synonyms

Tubulin beta-2A chain

Research area

Tags & cell markers; Signal transduction

Target protein

TUBB2A

Recommended dilution

Application	Dilution
IHC	1:50-300
IP	1:200
WB	1:50000-100000

Images

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1.Beta II tubulin Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.Beta II tubulin Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.Beta II tubulin Monoclonal antibody(Mix) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Western blot analysis of Mouse Brain tissue and Rat Brain tissue diluted at 1:100000.

1) Input: Mouse Brain Tissue Lysate 2) IP product: IP dilute 1: 200 Western blot analysis: primary antibody : TDY191 1: 10000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1: 5000

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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