Ki 67 Monoclonal Antibody(4A8)

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Home > Product > Antibody > Ki 67 Monoclonal Antibody(4A8)

Ki 67 Monoclonal Antibody(4A8)

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: IHC-P, IF, ICC

Product Summary

Size: 20ul,50ul,100ul

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: IHC-P, IF, ICC

Product overview

Full product name

Antigen KI-67

Code

BT-MCA0803

Host

Mouse

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Monoclonal

Immunogen

Synthetic Peptide of Ki 67

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Concentration

1 mg/ml

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for one year

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Uniprot accession

Human: P46013;

Molecular weight

358694

Gene ID

Human: 4288

Synonyms

MKI67; Antigen KI-67

Research area

Neuroscience; Cancer; Cell biology; Tags & cell markers

Target protein

MKI67

Recommended dilution

Application	Dilution
IF	1:50-200
IHC	1:200

Images

Immunofluorescence analysis of Hela cell. Annexin VI Polyclonal Antibody(green) was diluted at 1:200(4°C overnight). (red) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1.Ki 67 Monoclonal antibody(4A8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.Ki 67 Monoclonal antibody(4A8) was diluted at 1:200(4°C,overnight). 2.Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3.Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of Human-breast-cancer tissue. 1.Ki 67 Monoclonal antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Mouse-testis tissue. 1.Ki 67 Monoclonal antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of Rat-brain tissue. 1.Ki 67 Monoclonal antibody(4A8)(red) was diluted at 1:200(4°C,overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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