HDAC6(Phospho Ser22) Polyclonal Antibody

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Blood coagulation
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Home > Product > Antibody > HDAC6(Phospho Ser22) Polyclonal Antibody

HDAC6(Phospho Ser22) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Histone deacetylase 6

Code

BT-AP06589

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human HDAC6 around the phosphorylation site of Ser22. AA range:7-56

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Uniprot accession

Human: Q9UBN7; Mouse: Q9Z2V5;

Molecular weight

131431

Gene ID

Human: 10013; Mouse: 15185

Synonyms

Histone deacetylase 6; HDAC6; KIAA0901; JM21; Histone deacetylase 6; HD6

Research area

Epigenetics and nuclear signaling; Stem cells

Target protein

HDAC6

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 10000

Images

Immunofluorescence analysis of Hela cell. HDAC6 (phospho Ser22) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). Galectin-3 Monoclonal Antibody(6G2)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of HepG2 cells, using HDAC6 (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human testis, using HDAC6 (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of 3T3 cells using Phospho-HDAC6 (S22) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from NIH/3T3 cells treated with Anisomycin 25ug/ml 30', using HDAC6 (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

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Lead time: Within one week

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