IRS-1(phospho Ser636) Polyclonal Antibody

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Home > Product > Antibody > IRS-1(phospho Ser636) Polyclonal Antibody

IRS-1(phospho Ser636) Polyclonal Antibody

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 100ul,50ul,20ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Insulin receptor substrate 1

Code

BT-AP01000

Host

Rabbit

Isotype

IgG

Size

100ul,50ul,20ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636. AA range:603-652

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance.

Uniprot accession

Human: P35568; Mouse: P35569; Rat: P35570;

Molecular weight

131591

Gene ID

Human: 3667; Mouse: 16367; Rat: 25467

Synonyms

Insulin receptor substrate 1; IRS1; Insulin receptor substrate 1; IRS-1

Research area

Tags & Cell Markers

Target protein

IRS1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Transglutaminase 2 Antibody. The picture on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000

Western Blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000

Western Blot analysis of RAW using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000

Western blot analysis of 293T 3T3 lysis using Phospho-IRS-1 (S636) antibody. Antibody was diluted at 1:2000

Western blot analysis of lysates from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

100ul

$275.00
50ul

$180.00
20ul

$108.00

Add to Cart

Lead time: Within one week

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