PCNA Polyclonal Antibody

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Blood coagulation
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Cell biology
Signal transduction
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Stem cells
Epigenetics and nuclear signaling
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Home > Product > Antibody > PCNA Polyclonal Antibody

PCNA Polyclonal Antibody

Product Summary

Size: 20ul,50uL,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50uL,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Proliferating cell nuclear antigen

Code

BT-AP01367

Host

Rabbit

Isotype

IgG

Size

20ul,50uL,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Uniprot accession

Human: P12004; Mouse: P17918; Rat: P04961;

Molecular weight

28769

Gene ID

Human: 5111; Mouse: 18538; Rat: 25737

Synonyms

Proliferating cell nuclear antigen; PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin

Research area

Cancer; Cell biology; Epigenetics and nuclear signaling; Tags & cell markers

Target protein

PCNA

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 20000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PCNA Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.

Western Blot analysis of various cells using PCNA Polyclonal Antibody diluted at 1:2000

Western Blot analysis of COS7 cells using PCNA Polyclonal Antibody diluted at 1:2000

Western blot analysis of lysates from HepG2 cells, using PCNA Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Datasheet

Product size

Price

20ul

$108.00
50uL

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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