PP2A-C alpha(Phospho Tyr307) Polyclonal Antibody

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Home > Product > Antibody > PP2A-C alpha(Phospho Tyr307) Polyclonal Antibody

PP2A-C alpha(Phospho Tyr307) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Code

BT-AP07647

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human PP2A-alpha around the phosphorylation site of Tyr307. AA range:260-309

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Uniprot accession

Human: P67775; Mouse: P63330; Rat: P63331;

Molecular weight

35594

Gene ID

Human: 5515; Mouse: 19052; Rat: 24672

Synonyms

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C

Research area

Cancer; Signal transduction; Cell biology

Target protein

PPP2CA

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of Hela cell. PP2A-Cα (phospho Tyr307) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). HSP70 Monoclonal Antibody(3G10)(Green) was diluted at 1:200(4°C overnight).

Immunofluorescence analysis of A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of SKOV3 using p-PP2A-Cα (Y307) antibody. Antibody was diluted at 1:1000

Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Western blot analysis of lysates from A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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