Cdc25C(Phospho Ser216) Polyclonal Antibody

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Blood coagulation
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Cell biology
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Epigenetics and nuclear signaling
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Home > Product > Antibody > Cdc25C(Phospho Ser216) Polyclonal Antibody

Cdc25C(Phospho Ser216) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

M-phase inducer phosphatase 3

Code

BT-AP07499

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CDC25C around the phosphorylation site of Ser216. AA range:183-232

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

Uniprot accession

Human: P30307; Mouse: P48967;

Molecular weight

53365

Gene ID

Human: 995

Synonyms

M-phase inducer phosphatase 3; CDC25C; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C

Research area

Cell biology; Epigenetics and nuclear signaling

Target protein

CDC25C

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 10000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25C (Phospho-Ser216) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1:1000

Western Blot analysis of HuvEc cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HUVEC cells treated with serum 20% 30', using CDC25C (Phospho-Ser216) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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