FoxO1(Phospho Ser256) Polyclonal Antibody

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Home > Product > Antibody > FoxO1(Phospho Ser256) Polyclonal Antibody

FoxO1(Phospho Ser256) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Drosophila

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Drosophila

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Forkhead box protein O1

Code

BT-AP15648

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Uniprot accession

Human: Q12778; Mouse: Q9R1E0; Rat: G3V7R4;

Molecular weight

69662

Gene ID

Human: 2308; Mouse: 56458; Rat: 84482

Synonyms

Forkhead box protein O1; FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma

Research area

Cancer; Epigenetics and nuclear signaling; Metabolism; Neuroscience; Signal transduction

Target protein

FOXO1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of Hela cell. FoxO1 (phospho Ser256) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). Active Caspase-3 Monoclonal Antibody(5E1)(Green) was diluted at 1:200(4°C overnight).

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HeLa cells treated with EGF+Serum, using FKHR (Phospho-Ser256) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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