Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > Antibody > MEK-3(Phospho Ser218) Polyclonal Antibody

MEK-3(Phospho Ser218) Polyclonal Antibody

MEK-3(Phospho Ser218) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Dual specificity mitogen-activated protein kinase kinase 3

Code

BT-AP11255

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human MKK3 around the phosphorylation site of Ser189. AA range:173-222

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Uniprot accession

Molecular weight

39318

Gene ID

Human: 5606; Mouse: 26397

Synonyms

Dual specificity mitogen-activated protein kinase kinase 3; MAP2K3; MEK3; MKK3; PRKMK3; SKK2; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAPKK 3; MAPK/ERK kinase 3; MEK 3; Stress-activated protein kinase kinase 2; SAPK kinase 2; SAPKK-2; SAPKK2

Research area

Signal transduction; Cancer; Epigenetics and nuclear signaling; Immunology

Target protein

MAP2K3

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC-p 1: 100 - 1: 300
ELISA 1: 5000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MKK3 (Phospho-Ser189) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-MEK-3 (S218) Polyclonal Antibody
Western blot analysis of lysates from MDA-MB-435 cells, using MKK3 (Phospho-Ser189) Antibody. The lane on the left is blocked with the phospho peptide.

Protocols

Customer reviews and Q&As

No reviews
No reviews

Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

Add to Cart

Lead time: Within one week

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up