SDHA Monoclonal Antibody

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Recombinant proteins

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Blood coagulation
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Home > Product > Antibody > SDHA Monoclonal Antibody

SDHA Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA2795

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SDHA (AA: 516-665) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P31040: ;

Molecular weight

72.7KDa

Gene ID

6389

Synonyms

FP;PGL5;SDH1;SDH2;SDHF;CMD1GG;MC2DN1;NDAXOA

Research area

Tags & Cell Markers

Target protein

SDHA

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using SDHA mAb against human SDHA (AA: 516-665) recombinant protein. (Expected MW is 20 kDa)

Western blot analysis using SDHA mAb against HEK293-6e (1) and SDHA (AA: 516-665)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using SDHA mouse mAb against Jurkat (1), MCF-7 (2), Hela (3), HepG2 (4), PC-3 (5), .HL-60 (6),and NIH/3T3 (7) cell lysate.

Immunofluorescence analysis of Hela cells using SDHA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Raji cells using SDHA mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using SDHA mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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