ACOT4 Polyclonal Antibody

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Home > Product > Antibody > ACOT4 Polyclonal Antibody

ACOT4 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

ACOT4 Antibody

Code

BT-AP00188

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ACOT4. AA range:361-410

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Acyl-coenzyme A thioesterase 4 is an enzyme encoded by the ACOT4 gene in humans and is mapped to chromosome 14.In peroxisomes, it helps in β-oxidation of dicarboxylic acids to form succinate. ACOT4 is also responsible for the termination of β-oxidation of dicarboxylic acids of medium-chain length with the concomitant release of the corresponding free acids.

Uniprot accession

Human: Q8N9L9; Mouse: Q8BWN8;

Molecular weight

46327

Gene ID

Human: 122970

Synonyms

ACOT4; PTE2B; PTEIB; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib

Research area

Tags & Cell Markers; Cardiovascular

Target protein

ACOT4

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of various cells using ACOT4 Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from HeLa and COLO cells, using ACOT4 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of MCF7 cells, using ACOT4 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from COLO205 cells using ACOT4 antibody.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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