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Home > Product > Antibody > Actinin-Alpha1/2/3/4 Polyclonal Antibody

Actinin-Alpha1/2/3/4 Polyclonal Antibody

Actinin-Alpha1/2/3/4 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

Actinin-alpha1/2/3/4 Antibody

Code

BT-AP00216

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ACTN1/2/3/4. AA range:21-70

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.

Uniprot accession

Molecular weight

104854

Gene ID

Human: 87/88/89/81; Mouse: 109711/11472/11474/60595; Rat: 81634/63836

Synonyms

ACTN1; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN2; Alpha-actinin-2; Alpha-actinin skeletal muscle isoform 2; F-actin cross-li

Research area

Others

Target protein

ACTN1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
ELISA 1: 20000

Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western Blot analysis of various cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from 293, HeLa, and A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of A549 cells using Actinin-α1/2/3/4 Polyclonal Antibody diluted at 1:1000

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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