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Home > Product > Antibody > ATP5A Polyclonal Antibody

ATP5A Polyclonal Antibody

ATP5A Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

ATP5A Antibody

Code

BT-AP00733

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human ATP5A1. AA range:201-250

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

ATP5A1 encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5A1 encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Uniprot accession

Molecular weight

59751

Gene ID

Human: 498; Mouse: 11946; Rat: 65262

Synonyms

ATP5A1; ATP5A; ATP5AL2; ATPM; ATP synthase subunit alpha; mitochondrial

Research area

Tags & Cell Markers

Target protein

ATP5A1

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
ELISA 1: 40000

Images

Western blot analysis of the lysates from HT-29 cells using ATP5A1 antibody.
Western Blot analysis of various cells using ATP5A Polyclonal Antibody diluted at 1:500
Western Blot analysis of RAW264.7 cells using ATP5A Polyclonal Antibody diluted at 1:500
Western blot analysis of lysates from 293 and RAW264.7 cells, using ATP5A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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