Cytochrome b5 Polyclonal Antibody

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Home > Product > Antibody > Cytochrome b5 Polyclonal Antibody

Cytochrome b5 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Cytochrome b5 Antibody

Code

BT-AP02456

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Cytochrome b5 type A encoded by CYB5A is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in CYB5A are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for CYB5A.

Uniprot accession

Human: P00167; Mouse: P56395; Rat: P00173;

Molecular weight

15330

Gene ID

Human: 1528; Mouse: 109672; Rat: 64001

Synonyms

CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5

Research area

Signal Transduction; Metabolism

Target protein

CYB5A

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 5000

Images

Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody

Immunofluorescence analysis of NIH/3T3 cells, using CYB5 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, using CYB5 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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