PU.1 Polyclonal Antibody

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Home > Product > Antibody > PU.1 Polyclonal Antibody

PU.1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

PU.1 Antibody

Code

BT-AP07509

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SPI1. AA range:181-230

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

SPI1 encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. Spi-1 proto-oncogene can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this SPI1.

Uniprot accession

Human: P17947; Mouse: P17433; Rat: Q6BDS1;

Molecular weight

31083

Gene ID

Human: 6688; Mouse: 20375; Rat: 366126

Synonyms

SPI1; Transcription factor PU.1; 31 kDa-transforming protein

Research area

Immunology

Target protein

SPI1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 40000

Images

Western blot analysis of the lysates from HUVECcells using SPI1 antibody.

Western Blot analysis of various cells using PU.1 Polyclonal Antibody cells nucleus.

Western blot analysis of lysates from COS7 cells, using SPI1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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