Spindlin-1 Polyclonal Antibody

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Home > Product > Antibody > Spindlin-1 Polyclonal Antibody

Spindlin-1 Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Spindlin-1 Antibody

Code

BT-AP08508

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human SPIN1. AA range:111-160

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Sphingolipid transporter (By similarity). May be involved in necrotic or autophagic cell death.

Uniprot accession

Human: Q9Y657; Mouse: Q61142; Rat: Q4V8J7;

Molecular weight

29601

Gene ID

Human: 10927; Mouse: 20729; Rat: 1.00364e+008

Synonyms

SPIN1; OCR; SPIN; Spindlin-1; Ovarian cancer-related protein

Research area

Cell Biology

Target protein

SPIN1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ELISA	1: 20000

Images

Western Blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus.

Western blot analysis of lysates from HeLa cells, using SPIN1 Antibody. The lane on the right is blocked with the synthesized peptide.

Western Blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus.

Western blot analysis of the lysates from K562 cells using SPIN1 antibody.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of Hela cell. 1,Spindlin-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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