TFIIA- Alpha Polyclonal Antibody

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Home > Product > Antibody > TFIIA- Alpha Polyclonal Antibody

TFIIA- Alpha Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, ELISA

Product overview

Full product name

TFIIA-alpha Antibody

Code

BT-AP08951

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human TF2A1. AA range:281-330

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.

Uniprot accession

Human: P52655; Mouse: Q99PM3; Rat: O08949;

Molecular weight

41514

Gene ID

Human: 2957; Mouse: 83602; Rat: 83830

Synonyms

GTF2A1; TF2A1; Transcription initiation factor IIA subunit 1; General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42

Research area

Epigenetics and Nuclear Signaling

Target protein

GTF2A1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC	1: 100 - 1: 300
ELISA	1: 40000

Images

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of lysates from Jurkat and HUVEC cells, using TF2A1 Antibody. The lane on the right is blocked with the synthesized peptide.

Protocols

> Protocol Booklet

References

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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