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Home > Product > Antibody > TH Polyclonal Antibody

TH Polyclonal Antibody

TH Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

TH Antibody

Code

BT-AP08988

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Tyrosine Hydroxylase. AA range:10-59

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for one year

Background

Tyrosine 3-monooxygenase encoded by TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have be associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Uniprot accession

Molecular weight

58524

Gene ID

Human: 7054; Mouse: 21823; Rat: 25085

Synonyms

TH; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH

Research area

Neuroscience

Target protein

TH

Recommended dilution

Application Dilution
WB 1: 500 - 1: 2000
IHC 1: 100 - 1: 300
IF 1: 200 - 1: 1000
ELISA 1: 10000

Images

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Immunofluorescence analysis of HUVEC cells, using Tyrosine Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Tyrosine Hydroxylase Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from NIH/3T3/COLO cells, treated with Forskolin 40nM 30', using Tyrosine Hydroxylase Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using TH Polyclonal Antibody
Immunofluorescence analysis of Hela cell. 1,TH Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of 3T3 cells using TH Polyclonal Antibody

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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