Human Proprotein Convertase 9, PCSK9 Primacu™ ELISA Kit

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Home > Product > ELISA Primacu > Human Proprotein Convertase 9, PCSK9 Primacu™ ELISA Kit

Human Proprotein Convertase 9, PCSK9 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 206.5 pg/mL

Detection range: 468.75-30000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 206.5 pg/mL

Detection range: 468.75-30000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Proprotein convertase subtilisin/kexin type 9

Code

BPE112

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

206.5 pg/mL

Detection range

468.75-30000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

UniProt accession

Q8NBP7

MASS（Da）

74286

GeneID

255738

Synonyms

NARC1

Gene names

PCSK9

Research area

Signal transduction,Cardiovascular,Cell biology,Metabolism,Neuroscience,Stem cells

Target protein

PCSK9

Components

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

Protocol data

These standard curves of BPE112 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
30000 pg/ml	3.023	3.148	3.039
	3.272
15000 pg/ml	1.624	1.649	1.54
	1.673
7500 pg/ml	0.782	0.813	0.704
	0.843
3750 pg/ml	0.445	0.464	0.355
	0.482
1875 pg/ml	0.26	0.272	0.163
	0.283
937.5 pg/ml	0.169	0.181	0.072
	0.193
468.75 pg/ml	0.14	0.138	0.029
	0.136
0 pg/ml	0.112	0.109	0
	0.105

Precision

We measured random samples of BPE112 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	2674	134	5
Intra-assay	2	20	5690	221	3.9
Intra-assay	3	20	17781	808	4.5
Inter-assay	1	20	2692	266	9.9
Inter-assay	2	20	5707	569	10
Inter-assay	3	20	17915	1501	8.4

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

5*96T

BPE112

$2040.00
96T

BPE112

$480.00
15*96T

BPE112

$5400.00

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Lead time: Within two weeks

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