Product overview
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
| Standard Solution | 0.2mL × 1 vail |
| Dilution Buffer | 20mL × 1 vail |
| Stop Solution | 6mL × 1 vail |
| Substrate Solution A | 12mL × 1 vail |
| Substrate Solution B | 12mL × 1 vail |
| Wash Buffer Concentrate (25x) | 20mL × 1 vail |
| Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 10000 pg/ml | 2.281 | 2.394 | 2.278 |
| 2.506 | |||
| 5000 pg/ml | 1.298 | 1.306 | 1.19 |
| 1.313 | |||
| 2500 pg/ml | 0.711 | 0.735 | 0.619 |
| 0.758 | |||
| 1250 pg/ml | 0.393 | 0.403 | 0.287 |
| 0.412 | |||
| 625 pg/ml | 0.272 | 0.259 | 0.143 |
| 0.245 | |||
| 312.5 pg/ml | 0.18 | 0.174 | 0.058 |
| 0.167 | |||
| 156.25 pg/ml | 0.137 | 0.132 | 0.016 |
| 0.126 | |||
| 0 pg/ml | 0.112 | 0.116 | 0 |
| 0.12 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 20 | 429 | 25.8 | 6 |
| Intra-assay | 2 | 20 | 2044 | 73.3 | 3.6 |
| Intra-assay | 3 | 20 | 5111 | 234 | 4.6 |
| Inter-assay | 1 | 20 | 431 | 40.5 | 9.4 |
| Inter-assay | 2 | 20 | 2062 | 100 | 4.9 |
| Inter-assay | 3 | 20 | 5085 | 243 | 4.8 |
Protocols
References
Customer reviews and Q&As
