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Home > Product > ELISA Primacu > Mouse IFNAR1 Primacu™ ELISA Kit

Mouse IFNAR1 Primacu™ ELISA Kit

Mouse IFNAR1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 50.22 pg/mL

Detection range: 140.62-9000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 50.22 pg/mL

Detection range: 140.62-9000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Interferon alpha/beta receptor 1

Code

BPE323

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

50.22 pg/mL

Detection range

140.62-9000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

Interferon-alpha/beta receptor alpha chain (IFNAR1) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. Tyk2 slows down IFNAR1 degradation and that this is due, at least in part, to inhibition of IFNAR1 endocytosis. Mutant versions of IFNAR1, in which Tyr466 is changed to phenylalanine, can act in a dominant-negative manner to inhibit phosphorylation of STAT2. These observations are consistent with a model in which IFNAR1 mediates the interaction between JAK kinases and the STAT transcription factors.

UniProt accession

MASS(Da)

65796

GeneID

15975

Synonyms

Ifar;Ifnar

Gene names

Ifnar1

Research area

Epigenetics and nuclear signaling,Immunology,Signal transduction,Cancer

Target protein

IFNAR1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE323 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
9000 pg/ml 2.36 2.277 2.161
2.193
4500 pg/ml 1.24 1.303 1.187
1.365
2250 pg/ml 0.756 0.772 0.656
0.787
1125 pg/ml 0.444 0.439 0.323
0.434
562.5 pg/ml 0.283 0.284 0.168
0.284
281.25 pg/ml 0.19 0.196 0.08
0.201
140.625 pg/ml 0.157 0.154 0.038
0.15
0 pg/ml 0.111 0.116 0
0.121

Precision

We measured random samples of BPE323 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 336 17.4 5.2
Intra-assay 2 20 1682 67.4 4
Intra-assay 3 20 7010 312 4.5
Inter-assay 1 20 336 34.6 10.3
Inter-assay 2 20 1690 89.6 5.3
Inter-assay 3 20 7049 305 4.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE323

    $2320.00

  • 96T

    BPE323

    $580.00

  • 15*96T

    BPE323

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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